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Abe "three measures" to please the United States to get Okinawa Tags: topics for Category: Experimental proactive resolved
The purpose of the current biodosimetric radiation technology inherent delays and limitations of quantitative analysis and interpretation to determine the presence. We have discovered a new technology proactive has the advantage of direct measurement of circulating DNA by amplifying the inter-B1 region in the mouse genome, systemic plasma proactive DNA provides a sensitive method. Methods, materials, proactive real-time quantitative polymerase chain reaction (PCR) was used to detect by amplifying inter-B1 in plasma samples collected proactive from a total of 0-48 h in mice - or receiving partial-body 0 - 10 meter Yoo 137Csγ-ray DNA Genomic DNA level uniformity Yoo 1.86 6.5%) after; light irradiation / min. Results of the correlation coefficient and threshold levels between DNA (CT) is 0.996, the average recovery of DNA test was 87%. This article shows, BALB / c mice were exposed to radiation 10 Yoo Building (TBI), plasma DNA levels of radiation three hours after gradually increasing exercise, nine hours to reach the highest return to baseline within 48 hours. Or increase the plasma levels of DNA found in lower-torso upper-torso partial-body irradiation; however, these plasma DNA TBI dose at approximately the same level of increase doubled. This technique therefore reflects the cellular damage the body. Advantages of this article is, DNA extraction is not required, which is highly sensitive proactive detection, (0.002 ng) can be obtained good experimental results of plasma samples collected after 2.5 hours. Conclusion observed in the dose range from 2-10 Yoo deoxyribonucleic proactive acid (DNA) DNA dose-dependent increase in plasma radiation, suggesting that plasma biomarkers of radiation on the basis of existing and testing may be a useful adjunct.
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